The research need
Inflammatory pain involves diverse immune and sensory-neuron populations whose changing cellular states and interactions are difficult to resolve with bulk measurements.
Analysis contribution
Ashish Jain carried out the scRNA-seq data analysis, including quality control, integration, clustering, cell-type annotation, cell-cell communication analysis, downstream comparisons, and publication-ready figures.
Research impact
- Resolved immune-cell populations across zymosan, incision, and ultraviolet-burn pain models
- Characterized cell-type-specific transcriptional programs associated with pain and resolution
- Supported analysis of injury-specific neuroimmune interaction patterns
- Delivered reproducible results and publication-ready visualizations