The research need
Children receiving long-term parenteral nutrition can develop intestinal failure-associated liver disease, but the molecular processes driving its earliest stages remain poorly understood.
Analysis contribution
Ashish Jain carried out the bulk RNA-seq data analysis, including sequencing quality control, alignment and quantification, sample-level diagnostics, differential expression testing, and pathway-enrichment analysis comparing parenteral-nutrition and bottle-fed groups.
Research impact
- Detected broad transcriptomic differences between parenteral-nutrition and control liver tissue
- Identified 2,060 differentially expressed genes after quality control and statistical testing
- Revealed activation of inflammatory pathways and inhibition of cell-cycle and metabolic programs
- Supported prioritization of potential therapeutic strategies that may counteract disease-associated gene dysregulation