Analysis pipeline
Bulk RNA-seq analysis
From FASTQ quality control to differential expression and pathway-level insight.
Workflow
Six stages, one traceable analysis
- 01
FASTQ and metadata validation
- 02
Read quality control, alignment, or pseudoalignment
- 03
Gene-level quantification and sample quality control
- 04
Differential expression with an appropriate study design
- 05
GSEA, network, and pathway analysis
- 06
Reproducible report and publication-ready outputs
Built around the experiment—not a black box.
Tools and parameters are selected after reviewing study design, sample size, data quality, biological replicates, and the intended use of the results. Deliverables include methods documentation and analysis-ready outputs.
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